Biopesticides-an altenative to chemicals
Conventional Pesticides lead to several problems such as 25 million cases of acute occupational pesticide poisoning in developing countries each year . 14% of all known occupational injuries and 10% of all fatal injuries are caused by pesticides . Obsolete pesticides stored in developing countries – 20,000 tonnes in Africa alone. Fundamental component of Integrated Pest Management is the biopesticides which controls pests resistant toconventional pesticides. When used as a component of Integrated Pest Management (IPM) programs, biopesticides can greatly decrease the use of conventional pesticides, while crop yields remain high.Biopesticides are designed to affect only one specific pest or,in some cases, a few target organisms, in contrast to broadspectrum, conventional pesticides that may affect organisms as different as birds, insects, and mammals. Biopesticides often are effective in very small quantities and often decompose quickly, thereby resulting in lower exposures and largely avoiding the pollution problems caused by conventional pesticides. Biopesticides are pest management tools that are based on beneficial microorganisms (bacteria, viruses, fungi and protozoa), beneficial nematodes or other safe, biologically based active ingredients. Benefits of biopesticides include effective control of insects, plant diseases and weeds, as well as human and environmental safety. Biopesticides also play an important role in providing pest management tools in areas where pesticide resistance, niche markets, and environmental concerns limit the use of chemical pesticide products.Biopesticides are certain types of pesticides derived from such natural materials as animals, plants, bacteria, and certain minerals. For example, canola oil and baking soda have pesticidal applications and are considered biopesticides. At the end of 2001, there were approximately 195 registered biopesticide active ingredients and 780 products. Biopesticides fall into three major classes:
1. Microbial pesticides consist of a microorganism (e.g., a bacterium, fungus, virus or protozoan) as the active ingredient. Microbial pesticides can control many different kinds of pests, although each separate active ingredient is relatively specific for its target pest[s]. For example, there are fungi that control certain weeds, and other fungi that kill specific insects.
The most widely used microbial pesticides are subspecies and strains of Bacillus thuringiensis, or Bt. Each strain of this bacterium produces a different mix of proteins, and specifically kills one or a few related species of insect larvae. While some Bt’s control moth larvae found on plants, other Bt’s are specific for larvae of flies and mosquitoes. The target insect species are determined by whether the particular Bt produces a protein that can bind to a larval gut receptor, thereby causing the insect larvae to starve.
1. Plant-Incorporated-Protectants (PIPs) are pesticidal substances that plants produce from genetic material that has been added to the plant. For example, scientists can take the gene for the Bt pesticidal protein, and introduce the gene into the plant’s own genetic material. Then the plant, instead of the Bt bacterium, manufactures the substance that destroys the pest. The protein and its genetic material, but not the plant itself, are regulated by EPA.
2. Biochemical pesticides are naturally occurring substances that control pests by non-toxic mechanisms. Conventional pesticides, by contrast, are generally synthetic materials that directly kill or inactivate the pest. Biochemical pesticides include substances, such as insect sex pheromones, that interfere with mating, as well as various scented plant extracts that attract insect pests to traps. Because it is sometimes difficult to determine whether a substance meets the criteria for classification as a biochemical pesticide, EPA has established a special committee to make such decisions.
Advantages of using biopesticides:
ü Biopesticides are usually inherently less toxic than conventional pesticides.
ü Biopesticides generally affect only the target pest and closely related organisms, in contrast to broad spectrum, conventional pesticides that may affect organisms as different as birds, insects, and mammals.
ü Biopesticides often are effective in very small quantities and often decompose quickly, thereby resulting in lower exposures and largely avoiding the pollution problems caused by conventional pesticides.
ü When used as a component of Integrated Pest Management (IPM) programs, biopesticides can greatly decrease the use of conventional pesticides, while crop yields remain high.
ü To use biopesticides effectively, however, users need to know a great deal about managing pests.
ü Are slow acting.
ü Have relatively critical application times.
ü Suppress, rather than eliminate, a pest population.
ü Have limited field persistence and a short shelf life.
ü Are safer to humans and the environment than conventional pesticide.
ü Present no residue problems.
ü
S.No
TYPE OF CONTROL
EXAMPLES
Insect Control
Bacteria
Bacillus thuringiensis, B. sphaericus, Paenibacillus popilliae, Serratia entomophila
Viruses
nuclear polyhedrosis viruses, granulosis viruses, non-occluded baculoviruses
Fungi
Beauveria spp, Metarhizium, Entomophaga, Zoopthora, Paecilomyces fumosoroseus, Normuraea, Lecanicillium lecanii
Protozoa
Nosema, Thelohania, Vairimorpha
Entomopathogenic nematodes
Steinernemaspp,
Heterorhabditis spp
Others (not strictly biopesticides
Pheromones, parasitoids, predators, microbial by-products
Weed Control
Fungi
Colletotrichum gloeosporioides, Chondrostereum purpureum, Cylindrobasidium laeve
Bacteria
Xanthomonas campestris pv. Poannua
Plant Disease Control
Fungi
Ampelomyces quisqualis, Candida spp. , Clonostachys rosea f. catenulate, Coniothyrium minitans, Pseudozyma flocculosa, Trichoderma spp
Competitive inoculants
Bacillus pumilus, B. subtilis, Pseudomonas spp, Streptomyces griseoviridis
Composts, soil inoculants
Burkholderia cepacia
Nematicides etc.
Nematode trapping fungi
Myrothecium verrucaria, Paecilomyces lilacinus
Bacteria
Bacillus firmus, Pasteuria penetrans
Mollusc parasitic nematode
Phasmarhabditis hermaphrodita
Biopesticide and Bioweapons
Developments in biotechnology makes it possible to greatly amplify the impact of traditional biowarfare agents and made the means to create bio-terror weapons available to a significant part of the population through training in genetic engineering. There is an extensive history of the use of bio-warfare agents, and in recent years, bioterrorism has been a growing concern. An extensive biological warfare program in Iraq was discovered after the Gulf War of 1991. Revelations concerning the covert program in the former Soviet Union also attracted much public attention. The Rajneeshee Cult, an Indian religious group, contaminated restaurant salad bars in Oregon in 1984 with Salmonella typhimurium, and about 751 citizens were infected. The cult’s motivation was to incapacitate voters in order to win a local election and to seize political control of Dallas and Wasco counties. Larry Wayne Harris wanted to alert Americans to the Iraqi biological warfare threat and sought a separate homeland for whites in the United States. He had links to Christian Identity and the Aryan Nation, a white supremacist group. Harris made vague threats against US federal officials on behalf of right-wing “patriot” groups. He obtained the B. anthracis vaccine strain and Yersinia pestis (plague bacteria), and reportedly, several other bacteria, and discussed the dissemination of biological warfare agents by means of crop duster aircraft and other methods. Harris was arrested in 1998 after he made threatening remarks to US officials and talked openly about biological warfare terrorism .
Bacillus anthracis the cause of anthrax poisoning is currently a great concern because of its employment as a terror weapon. Bacillus thuringiensis is both a major pesticide and the source of the genes used to produce insect toxins in GM crops. A third bacterium, Bacillus cereus, is a common soil bacterium and a common cause of food poisoning. The three species of bacteria are closely related, differing mainly in their plasmids (plasmids are circular DNA molecules that contain genetic origins of replication that allow them to replicate independently of the chromosome). The plasmids of the three species may readily be transferred from one species to another . The toxin genes from the three species are located on the plasmids and the genes tend to cluster in ‘islands’ that sometimes are mobilized (caused to move) by lysogenic bacterial viruses (bacteriophages that integrate themselves into the bacterial genome or bacterial plasmid as prophage). The ready exchange of plasmids bearing toxin genes between the three species has raised some concern .The virulence of B. anthracis depends on the presence of two large plasmids, strains lacking one or the other plasmid are not virulent. Plasmid X02 carries genes that make polymers of glutamic acid (one of twenty amino acids that make proteins). These glutamic acid polymers go on the cell surface to inhibit phagocytes, cells in the body that engulf and take in bacteria and digest them. Plasmid X01 carries the three toxin genes coding for edema factor, lethal factor and protective antigen .
The insect killing ability of B. thuringiensis is based on the presence of an island of toxin genes on one of many (up to 17) plasmids in the bacterium . The strain, B. thuringiensis serovar israelensis, has a plasmid-borne prophage that is induced to multiply when the strain mates with phage-insensitive strains of B. thuringiensis or B. cereus . The endotoxins of B. thuringiensis (bt toxins) are stored as inactive crystals in bacterial spores, which are activated in the insect gut to create pores on the cells of the insect gut, causing an inrush of water that bursts the cell. In the event that B. anthracis mated to transfer plasmids to B. thuringiensis, recombination could create plasmids bearing toxins both for anthrax and for killing insects. New strains of B. anthracis with unpredictable properties could arise.
The bt toxin genes are employed in crop genetic engineering. Currently, there has been little or no effort to evaluate the possible recombination between B. anthracis in the field and the endotoxin genes of crop plants. Such gene exchange could occur in the soil between GM plant debris and bacteria.Also, it is not unlikely that GM crops carrying anthrax genes could be produced either for vaccines or for bio-weapons.
Harpin technology for the Third World
Eden BioSciences has unveiled a program to make Messenger®available to less-developed countries. It will be made available in thefirst instance to small-scale farmers in Ethiopia and Kenya. Within ayear, the project will be expanded to other African and EasternEuropean countries that apply to Eden for inclusion in the project.Representatives from Eden recently gave a half-day presentation onthe Subsistence Farming Project to an audience which includedsome 20 ministers of agriculture from Eastern European and Africannations.
Eden BioScience Corp. has received a Presidential Green ChemistryChallenge Award (Small Business Category) for its technicalinnovation in the development of harpin technology. These awardsare given annually through the EPA Office of Pollution Preventionand Toxics to recognize organizations and individuals that are successfully researching, developing, and implementing outstanding green chemical technologies. The latter are defined as chemical products and manufacturing processes that reduce or eliminate theuse or generation of hazardous substances. The award waspresented at the National Academy of Sciences by Stephen L.Johnson, EPA Assistant Administrator for Prevention, Pesticides, and Toxic Substances, during the fifth National Green Chemistry and Engineering Conference. This is only the third time an agriculturalproduct company has won the award.The harpin formulation Messenger® is a watersoluble,granular powder that is topically applied either independentlyor in conjunction with traditional chemical pesticides.Once applied, Messenger® degrades rapidly and leaves nodetectable residue on plants or in the soil and degrades rapidly in the environment; there is therefore no risk of contamination of ground or surface water. In addition, harpin does not alter the plant’s DNA.
Unlike traditional chemical pesticides, harpin does not kill or otherwise adversely affect pests or pathogens, and hence it does not exert the selection pressure that promotes the development of resistance in pest populations, thus reducing the likelihood of resistance or cross-resistance development. Harpin is ideally suited to controlling pests that have developed resistance to conventional chemical treatments and to being used as a partner with highly pest-specific,lower risk products.
The harpin formulation Messenger® has been extensively tested in more than 1000 field trials, on more than 40 crop groupings plus turf and ornamentals throughout the world .It has been shown to be effective on a wide variety of economically important crops, such as cotton, wheat,cucumber, citrus, tobacco, strawberry, tomato and peppers. Messenger® has demonstrated effective control of viral plant diseases for which there are currently no chemicalcontrols. Notable examples include tobacco and cucumber mosaic viruses in tomato and pepper, tobacco mosaic virusin tobacco, and beet curly-top virus in peppers. Messenger®-treated tomato plants also exhibit reducedgalling (root nodules), “tolerance” to root-knot nematodes,and/or increased volume and grade of marketable fruit. Additional benefits in Messenger®-treated tobacco include a“tolerance” to cyst nematodes. It has also demonstrated effective management of other soil-borne pathogens, such asFusarium spp. in tomato, cucumber, strawberry and wheat.
Use in IPM programmes
Messenger®’s broad spectrum of control, ease of use and compatibility with established Integrated Pest Mangement IPM) practices make it an ideal product for IPM programs. It can be used throughout the growing season as a crop production tool that induces disease resistance and in sectrepellence, while at the same time promoting optimal plant health. It does not disrupt the natural or introduced populations of beneficial predators and parasites that are often an integral component of IPM.
Potential of Biopesticide
The efficacy of many of the biopesticide can equal that of conventional chemical pesticides.However, the mode of action will be different.With many of the biopesticides, the time from exposure to morbidity and death of the target insect may be 2 to 10 days. Understanding the fundamental differences in the mode of action of biopesticide vs. traditional pesticides is important since the use patterns of a biopesticide may be different from traditional pesticides to control a particular pest species.It is important to be careful when using any pesticide, even organic or natural or biopesticide.
Even if this product is considered to be organic in origin, it is still a pesticide. Just because a product is though to be organic, or natural, does not mean that it is not toxic. Some organic pesticides are as toxic, or even more toxic, than many synthetic chemical pesticides. Organic pesticides have specific modes of action, just as do synthetic pesticides have specific modes of action, just asdo synthetic pesticides. While some organic pesticides may be nontoxic or are only slightly toxic to people, they may be very toxic to other animals. For instance, the organic pesticide ryania is very toxic to fish. Also, some organic pesticides may be toxic to beneficial insects, such as honeybees, if they are combined with other materials, such as combining pyrethrins with rotenone. The use of an Integrated Pest Management Program(IPM) is important to insure success.
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Tags: biopesticides, chemical, chemicals, environment, environmental, farming, green, green chemistry, insect, insect pests, insects, integrated pest management, microbial pesticides, organic, organic pesticide, organic pesticides, pest management, pesticide, pesticides, pests, products
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